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《PEACE MAKER 铁 ~和平捍卫队 铁~》以群雄割据、时代动乱的日本幕府末期作为背景，主角市村铁之助（小铁）的父亲是个崇洋的新派人士，并以作为乱世中的 PEACE MAKER 自许，后来小铁的双亲被攘夷派格杀，此事在小铁心中烙下阴影，并立志要变强以报双亲之仇当他终于如愿以偿加入人人畏惧的「新撰组」，好笑又紧凑的故事就此展开……。
而最新动画电影的监督为 竹内浩志、系列构成为 梅原英司，动画制作公司是 WHITE FOX，参与配音演出的声优包括 梶裕贵（饰演主角铁之助，少年期由小林由美子配音演出）、樱井孝宏（饰演山崎烝）、中田让治（饰演土方岁三）、斋贺光希（饰演沖田总司）。